HPRT1 Antibody / HGPRT1

Hypoxanthine-guanine phosphoribosyltransferase is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000

UniProt

P00492

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 150-178 from the human protein was used as the immunogen for the HPRT1 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HPRT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HPRT1 antibody is available for research use only.

Storage Conditions

Aliquot the HPRT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the HPRT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.