Cytochrome p450 2C19 Antibody / CYP2C19

CYP2C19 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, omeprazole, diazepam and some barbiturates. Polymorphism within its gene is associated with variable ability to metabolize mephenytoin, known as the poor metabolizer and extensive metabolizer phenotypes.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

P33261

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 82-108 from the human protein was used as the immunogen for the Cytochrome p450 2C19 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, WB, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Cytochrome p450 2C19 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Cytochrome p450 2C19 antibody is available for research use only.

Storage Conditions

Aliquot the Cytochrome p450 2C19 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Cytochrome p450 2C19 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human hepatocellular carcinoma tissue with Cytochrome p450 2C19 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.