TYSY Antibody / Thymidylate Synthase

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

P04818

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 265-294 from the human protein was used as the immunogen for the TYSY antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TYSY antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TYSY antibody is available for research use only.

Storage Conditions

Aliquot the TYSY antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the TYSY antibody may be required due to differences in protocols and secondary/substrate sensitivity.