ETHE1 Antibody

ETHE1 is a sulfur dioxygenase that localizes within the mitochondrial matrix. The enzyme functions in sulfide catabolism. Mutations in its gene result in ethylmalonic encephalopathy.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells)

UniProt

O95571

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 103-130 from the human protein was used as the immunogen for the ETHE1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ETHE1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ETHE1 antibody is available for research use only.

Storage Conditions

Aliquot the ETHE1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ETHE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human colon carcinoma tissue with ETHE1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.