EEF1A1/2 Antibody

EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000

UniProt

P68104

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 361-389 from the human EEF1A1 protein was used as the immunogen for the EEF1A1/2 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IF, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the EEF1A1/2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This EEF1A1/2 antibody is available for research use only.

Storage Conditions

Aliquot the EEF1A1/2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the EEF1A1/2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.