EEF1A1/2 Antibody
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000
UniProt
P68104
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 361-389 from the human EEF1A1 protein was used as the immunogen for the EEF1A1/2 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, IF, IHC-P, WB
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the EEF1A1/2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This EEF1A1/2 antibody is available for research use only.
Storage Conditions
Aliquot the EEF1A1/2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic, nuclear
Image Legend
Western blot testing of human MCF7 cell lysate with EEF1A1/2 antibody. Predicted molecular weight ~50 kDa.
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