GZMB Antibody / Granzyme B

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

P10144

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 175-203 from the human protein was used as the immunogen for the GZMB antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, WB, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the GZMB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GZMB antibody is available for research use only.

Storage Conditions

Aliquot the GZMB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the GZMB antibody may be required due to differences in protocols and secondary/substrate sensitivity.