ARPC1A Antibody

ARPC1A is one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000

UniProt

Q92747

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 286-315 from the human protein was used as the immunogen for the ARPC1A antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IF, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ARPC1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ARPC1A antibody is available for research use only.

Storage Conditions

Aliquot the ARPC1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ARPC1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human skeletal muscle tissue with ARPC1A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.