Major Vault Protein Antibody / MVP

MVP, the major vault protein, is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000

UniProt

Q14764

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 863-893 from the human protein was used as the immunogen for the Major Vault Protein antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, FACS, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Major Vault Protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Major Vault Protein antibody is available for research use only.

Storage Conditions

Aliquot the Major Vault Protein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Major Vault Protein antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human lung carcinoma tissue with Major Vault Protein antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.