ILKAP Antibody

Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

Q9H0C8

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 243-270 from the human protein was used as the immunogen for the ILKAP antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ILKAP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ILKAP antibody is available for research use only.

Storage Conditions

Aliquot the ILKAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ILKAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human skeletal muscle tissue with ILKAP antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.