DNAJA1 Antibody / HDJ2

DnaJ homolog subfamily A member 1 plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

P31689

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 294-323 from the human protein was used as the immunogen for the DNAJA1 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the DNAJA1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This DNAJA1 antibody is available for research use only.

Storage Conditions

Aliquot the DNAJA1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the DNAJA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human prostate carcinoma tissue with DNAJA1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.