FGFBP3 Antibody / Fibroblast growth factor-binding protein 3

Heparin-binding protein which binds to FGF2, prevents binding of FGF2 to heparin and probably inhibits immobilization of FGF2 on extracellular matrix glycosaminoglycans, allowing its release and subsequent activation of FGFR signaling which leads to increased vascular permeability. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

Q8TAT2

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 181-210 from the human protein was used as the immunogen for the FGFBP3 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the FGFBP3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This FGFBP3 antibody is available for research use only.

Storage Conditions

Aliquot the FGFBP3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the FGFBP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human brain tissue with FGFBP3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.