STEA2 Antibody

This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro. Increased transcriptional expression of the human gene is associated with prostate cancer progression. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

Q8NFT2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 229-258 from the human protein was used as the immunogen for the STEA2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the STEA2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This STEA2 antibody is available for research use only.

Storage Conditions

Aliquot the STEA2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the STEA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of human HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide, human HepG2 cell lysate in the 3) absence and 4) presence of immunizing peptide, and mouse spleen tissue lysate in the 5) absence and 6) presence of immunizing peptide with STEA2 antibody. Predicted molecular weight: 48-56 kDa (3 isoforms) .