Pyruvate Dehydrogenase E2 Antibody / DLAT
DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC) . PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC) . In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000
UniProt
P10515
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 579-607 from the human protein was used as the immunogen for the Pyruvate Dehydrogenase E2 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, IHC-P, WB
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Pyruvate Dehydrogenase E2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Pyruvate Dehydrogenase E2 antibody is available for research use only.
Storage Conditions
Aliquot the Pyruvate Dehydrogenase E2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic
Image Legend
Western blot testing of human 1) LNCaP and 2) MCF7 cell lysate with Pyruvate Dehydrogenase E2 antibody. Predicted molecular weight ~69 kDa.
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