Inosine triphosphate pyrophosphatase Antibody / ITPA / ITPase

ITPA hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. The encoded protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency. Two transcript variants encoding two different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

Q9BY32

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 24-51 from the human protein was used as the immunogen for the Inosine triphosphate pyrophosphatase antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Inosine triphosphate pyrophosphatase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Inosine triphosphate pyrophosphatase antibody is available for research use only.

Storage Conditions

Aliquot the Inosine triphosphate pyrophosphatase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Inosine triphosphate pyrophosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) A375, 2) HeLa, 3) HepG2, 4) mouse brain and 5) rat liver lysate with Inosine triphosphate pyrophosphatase antibody. Predicted molecular weight ~21 kDa.