Phospho-MeCP2 Antibody (pS423)

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD) . Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500

UniProt

P51608

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding the amino acids surrounding phosphorylated S423 was used as the immunogen for the phospho-MeCP2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-MeCP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-MeCP2 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-MeCP2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the phospho-MeCP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of lysate from human Jurkat cells treated or non-treated with CIP/CIAP (calf intestinal alkaline phosphatase) with phospho-MeCP2 antibody. Commonly observed molecular weights: ~55 kDa and ~75 kDa.