Phospho-CDK7 Antibody (pT170)

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK) . It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:50-1:100, Dot blot: 1:500, Western blot: 1:500-1:1000

UniProt

P50613

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding the amino acids surrounding phosphorylated T170 was used as the immunogen for the phosph-CDK7 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, DB, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-CDK7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-CDK7 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-CDK7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the phospho-CDK7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of lysate from mouse NIH 3T3 cells treated or non-treated with lambda protein phosphatase with phospho-CDK7 antibody. Expected molecular weight ~39 kDa.