HNRPL Antibody / hnRNP L

HNRPL is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. L is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

P14866

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 249-277 from the human protein was used as the immunogen for the HNRPL antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HNRPL antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HNRPL antibody is available for research use only.

Storage Conditions

Aliquot the HNRPL antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the HNRPL antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human hepatocellular carcinoma tissue with HNRPL antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.