IMMT Antibody / MIC60

Mitochondria are the center of cellular energy production and essential metabolic reactions. As double membrane-bound organelles, mitochondria from different species, tissues, and metabolic states are highly polymorphic in nature, yet exhibit common structural features. The ultrastructural variations in mitochondrial architecture occur mainly due to the differences in the amount and shape of cristae. Abundant cristae are found in mitochondria from tissues where energy demand is high. Analysis of the human heart mitochondrial proteome shows that mitofilin is one of the most abundant mitochondrial proteins. It appears to play an important role in the maintenance of cristae morphology.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000

UniProt

Q16891

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 700-729 from the human protein was used as the immunogen for the IMMT antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, FACS, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the IMMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IMMT antibody is available for research use only.

Storage Conditions

Aliquot the IMMT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the IMMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human colon carcinoma tissue with IMMT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.