PDIA3 Antibody / Protein disulfide-isomerase A3 / ERp57

PDIA3 is the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

P30101

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 192-220 from the human protein was used as the immunogen for the PDIA3 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PDIA3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PDIA3 antibody is available for research use only.

Storage Conditions

Aliquot the PDIA3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PDIA3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human lung carcinoma tissue with PDIA3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.