TUFM Antibody

TUFM is a protein which participates in protein translation in mitochondria. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

P49411

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 75-104 from the human protein was used as the immunogen for the TUFM antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TUFM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TUFM antibody is available for research use only.

Storage Conditions

Aliquot the TUFM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the TUFM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human hepatocellular carcinoma tissue with TUFM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.