Dimethylargininase 1 Antibody / DDAH1

DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Western blot: 1:500-1:1000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

O94760

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 221-250 from the human protein was used as the immunogen for the Dimethylargininase 1 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, WB, FACS, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Dimethylargininase 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Dimethylargininase 1 antibody is available for research use only.

Storage Conditions

Aliquot the Dimethylargininase 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Dimethylargininase 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE mouse kidney tissue with Dimethylargininase 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.