Adenine phosphoribosyltransferase Antibody / APRT

A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP) . It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

P07741

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 12-40 from the human protein was used as the immunogen for the Adenine phosphoribosyltransferase antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Adenine phosphoribosyltransferase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Adenine phosphoribosyltransferase antibody is available for research use only.

Storage Conditions

Aliquot the Adenine phosphoribosyltransferase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Adenine phosphoribosyltransferase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human hepatocellular carcinoma tissue with Adenine phosphoribosyltransferase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.