TCP1 gamma Antibody / CCT3

CCT3 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC) . This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:1000

UniProt

P49368

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 298-326 from the human protein was used as the immunogen for the TCP1 gamma antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TCP1 gamma antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TCP1 gamma antibody is available for research use only.

Storage Conditions

Aliquot the TCP1 gamma antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the TCP1 gamma antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human lung carcinoma tissue with TCP1 gamma antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.