Alpha Enolase Antibody / ENOA

ENOA, or ENO1, is one of three enolase isoenzymes found in mammals; the protein alpha-enolase, a homodimeric soluble enzyme, and is also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

P06733

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 405-434 from the human protein was used as the immunogen for the Alpha Enolase antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, IF, FACS

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Alpha Enolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Alpha Enolase antibody is available for research use only.

Storage Conditions

Aliquot the Alpha Enolase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Alpha Enolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of 1) human Jurkat, 2) human MCF7, 3) human U-251 and 4) mouse brain lysate with Alpha Enolase antibody. Predicted molecular weight ~47 kDa.