HSPA8 Antibody / HSC70

HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. The protein also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25

UniProt

P11142

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 82-110 from the human protein was used as the immunogen for the HSPA8 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IF, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HSPA8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HSPA8 antibody is available for research use only.

Storage Conditions

Aliquot the HSPA8 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the HSPA8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of 1) human A431, 2) human HeLa, 3) mouse NIH 3T3 and 4) rat H-4-II-E cell lysate with HSPA8 antibody. Expected molecular weight: 70-73 kDa.