PA2G4 Antibody / EBP1 / ErbB3-binding protein 1

PA2G4 is an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. The protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. It is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. It has been implicated in growth inhibition and the induction of differentiation of human cancer cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:1000

UniProt

Q9UQ80

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 228-255 from the human protein was used as the immunogen for the PA2G4 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PA2G4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PA2G4 antibody is available for research use only.

Storage Conditions

Aliquot the PA2G4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PA2G4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human lung carcinoma tissue with PA2G4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.