RFC5 Antibody

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000

UniProt

P40937

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 170-200 from the human protein was used as the immunogen for the RFC5 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the RFC5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This RFC5 antibody is available for research use only.

Storage Conditions

Aliquot the RFC5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the RFC5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.