MTHFD1 Antibody / C-1-tetrahydrofolate synthase

MTHFD1 is a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

P11586

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 535-562 from the human protein was used as the immunogen for the MTHFD1 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, WB, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the MTHFD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MTHFD1 antibody is available for research use only.

Storage Conditions

Aliquot the MTHFD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the MTHFD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.