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ZIC3 Antibody

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:50-1:100 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

O60481

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 239-267 from the human protein was used as the immunogen for the ZIC3 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ZIC3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ZIC3 antibody is available for research use only.

Storage Conditions

Aliquot the ZIC3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ZIC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of human HL60 cell lysate with ZIC3 antibody. Predicted molecular weight ~51 kDa.

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