SUMO-activating enzyme subunit 1 Antibody / SAE1

The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:25

UniProt

Q9UBE0

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 300-329 from the human protein was used as the immunogen for the SUMO-activating enzyme subunit 1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the SUMO-activating enzyme subunit 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SUMO-activating enzyme subunit 1 antibody is available for research use only.

Storage Conditions

Aliquot the SUMO-activating enzyme subunit 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the SUMO-activating enzyme subunit 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.