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NUB1 Antibody / NEDD8 ultimate buster 1 / NYREN18

NUB1/NYREN18 is a specific down-regulator of the NEDD8 conjugation system. It recruits NEDD8 and its conjugates to the proteasome for degradation. It also interacts with PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:25

UniProt

Q9Y5A7

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 87-117 from the human protein was used as the immunogen for the NYREN18 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the NYREN18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NYREN18 antibody is available for research use only.

Storage Conditions

Aliquot the NYREN18 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the NYREN18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

Western blot testing of mouse liver lysate with NUB1 antibody. Expected molecular weight: 70-100 kDa depending on level of ubiquitination.

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