Ufm1-conjugating enzyme 1 Antibody / Ufc1

Various ubiquitin-like (UBL) post-translational modifiers contribute critically to the protein degradation pathway . These UBLs are covalently linked to most target proteins through an enzymatic cascade analogous to ubiquitylation, consisting of E1 (activating), E2 (conjugating), and E3 (ligating) enzymes. Ufc1 Belongs to the ubiquitin-conjugating enzyme family, and is an E2-like enzyme which forms an intermediate with UFM1, a novel ubiquitin-fold modifier, via thioester linkage.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells)

UniProt

Q9Y3C8

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

An amino acid sequence from the C-terminal region of the human protein was used as the immunogen for the Ufm1-conjugating enzyme 1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Ufm1-conjugating enzyme 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Ufm1-conjugating enzyme 1 antibody is available for research use only.

Storage Conditions

Aliquot the Ufm1-conjugating enzyme 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Ufm1-conjugating enzyme 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human bladder carcinoma tissue with Ufm1-conjugating enzyme 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.