Med12 Antibody

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:25

UniProt

A2AGH6

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 1991-2025 from the mouse protein was used as the immunogen for the Med12 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Med12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Med12 antibody is available for research use only.

Storage Conditions

Aliquot the Med12 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Med12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.