Trim24 Antibody / Tif1 alpha / Tif1a
Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac) (By similarity) . Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis via its effects on p53/TP53 levels. Up-regulates ligand- dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, such as RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. Required for normal transition from proliferating neonatal hepatocytes to quiescent adult hepatocytes.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25
UniProt
Q64127
Host
Rabbit
Reactivity
Mouse
Immunogen
A portion of amino acids 789-843 from the mouse protein was used as the immunogen for the Trim24 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IF, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Trim24 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Trim24 antibody is available for research use only.
Storage Conditions
Aliquot the Trim24 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic, nuclear
Image Legend
Western blot testing of mouse 1) NIH 3T3, 2) spleen, 3) testis and 4) thymus lysate with Trim 24 antibody. Expected molecular weight: 117-140 kDa.
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