Brachyury Antibody / Protein T / TBXT
The protein encoded by the TBXT gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Immunofluorescence: 1:25
UniProt
O15178
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 15-43 from the human protein was used as the immunogen for the Brachyury antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IF
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Brachyury antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Brachyury antibody is available for research use only.
Storage Conditions
Aliquot the Brachyury antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Nuclear
Image Legend
Western blot testing of human 1) A431 and 2) NCI-H460 cell lysate with Brachyury antibody. Predicted molecular weight ~47 kDa.
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