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TOPK Antibody / PBK

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

Q9JJ78

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 58-86 from the mouse protein was used as the immunogen for the TOPK antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IHC-P, WB, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TOPK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TOPK antibody is available for research use only.

Storage Conditions

Aliquot the TOPK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the TOPK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

IHC testing of FFPE rat testis tissue with TOPK antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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