PI3KC3 Antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole) . The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Immunofluorescence: 1:25

UniProt

Q8NEB9

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 14-39 from the human protein was used as the immunogen for the PI3KC3 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, WB, IF

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PI3KC3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PI3KC3 antibody is available for research use only.

Storage Conditions

Aliquot the PI3KC3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PI3KC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.