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ALKBH5 Antibody

Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

Q6P6C2

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 302-330 from the human protein was used as the immunogen for the ALKBH5 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ALKBH5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ALKBH5 antibody is available for research use only.

Storage Conditions

Aliquot the ALKBH5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ALKBH5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

Western blot testing of human HEK293 cell lysate with ALKBH5 antibody. Predicted molecular weight: ~52 kDa.

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