Rubicon Antibody / RUBCN / BARON

Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25

UniProt

Q92622

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 241-271 from the human protein was used as the immunogen for the Rubicon antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P, IF

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Rubicon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Rubicon antibody is available for research use only.

Storage Conditions

Aliquot the Rubicon antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Rubicon antibody may be required due to differences in protocols and secondary/substrate sensitivity.