Rubicon Antibody / RUBCN / BARON
Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25
UniProt
Q92622
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 241-271 from the human protein was used as the immunogen for the Rubicon antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, IHC-P, IF
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Rubicon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Rubicon antibody is available for research use only.
Storage Conditions
Aliquot the Rubicon antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic
Image Legend
Western blot testing of human 1) K562 and 2) HEK293 cell lysate with Rubicon antibody. Expected molecular weight ~130 kDa.
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