SIGLEC5 Antibody

SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differenciation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25

UniProt

O15389

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 8-39 from the human protein was used as the immunogen for the SIGLEC5 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the SIGLEC5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SIGLEC5 antibody is available for research use only.

Storage Conditions

Aliquot the SIGLEC5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the SIGLEC5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.