CCR4-NOT transcription complex subunit 4 Antibody / CNOT4

CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective. The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25

UniProt

O95628

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Recombinant human protein was used as the immunogen for the CCR4-NOT transcription complex subunit 4 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CCR4-NOT transcription complex subunit 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CCR4-NOT transcription complex subunit 4 antibody is available for research use only.

Storage Conditions

Aliquot the CCR4-NOT transcription complex subunit 4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CCR4-NOT transcription complex subunit 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of human 1) MCF7, 2) K562 and 3) HEK293 cell lysate with CCR4-NOT transcription complex subunit 4 antibody. Expected molecular weight: 48-84 kDa (multiple isoforms) .