C-Raf Antibody / Raf1

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3 (By similarity) .

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000

UniProt

Q99N57

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 86-113 from the mouse protein was used as the immunogen for the c-Raf antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, FACS, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the c-Raf antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This c-Raf antibody is available for research use only.

Storage Conditions

Aliquot the c-Raf antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the c-Raf antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE mouse skeletal muscle tissue with c-Raf antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.