Pyruvate dehydrogenase kinase 2 Antibody / PDK2

Pdk2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

Q9JK42

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 78-107 from the mouse protein was used as the immunogen for the Pyruvate dehydrogenase kinase 2 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Pyruvate dehydrogenase kinase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Pyruvate dehydrogenase kinase 2 antibody is available for research use only.

Storage Conditions

Aliquot the Pyruvate dehydrogenase kinase 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Pyruvate dehydrogenase kinase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE mouse heart tissue with Pyruvate dehydrogenase kinase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.