DYRK2 Antibody

Role in the regulation of cellular growth and/or development. Regulates TP53 by phosphorylation on Ser-46 to induce apoptosis in response to DNA damage, functioning downstream of ATM. Inactivates GYS1 by phosphorylation at Ser-641, and potentially also a second phosphorylation site, thus regulating glycogen synthesis. Phosphorylates EIF2B5 at Ser-544, enabling its subsequent phosphorylation and inhibition by GSK3, and may play a more general role in the priming of GSK3 substrates (By similarity) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25

UniProt

Q5U4C9

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 521-549 from the mouse protein was used as the immunogen for the DYRK2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the DYRK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This DYRK2 antibody is available for research use only.

Storage Conditions

Aliquot the DYRK2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the DYRK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of 1) human HeLa, 2) human HepG2, 3) human SK-OV-3 and 4) mouse C2C12 cell lysate with DYRK2 antibody. Predicted molecular weight ~67 kDa.