Splicing factor 3B subunit 1 Antibody / SF3B1

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP) . The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

O75533

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 374-402 from the human protein was used as the immunogen for the Splicing factor 3B subunit 1 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Splicing factor 3B subunit 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Splicing factor 3B subunit 1 antibody is available for research use only.

Storage Conditions

Aliquot the Splicing factor 3B subunit 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Splicing factor 3B subunit 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

Western blot testing of 1) human PANC-1, 2) human HeLa, 3) human Jurkat, 4) mouse NIH 3T3, 5) human H-4-II-E, 6) human A431 and 7) human HepG2 cell lysate with Splicing factor 3B subunit 1 antibody. Predicted molecular weight ~146 kDa.