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Gastric Intrinsic Factor Antibody / GIF / CBLIF

This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

P27352

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 189-218 from the human protein was used as the immunogen for the Gastric Intrinsic Factor antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

FACS, IF, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Gastric Intrinsic Factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Gastric Intrinsic Factor antibody is available for research use only.

Storage Conditions

Aliquot the Gastric Intrinsic Factor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Gastric Intrinsic Factor antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human K562 cell lysate with Gastric Intrinsic Factor antibody. Predicted molecular weight ~45 kDa.

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