Single-minded homolog 1 Antibody / SIM1

SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis, it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or mental retardation of Down syndrome.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

P81133

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for the Single-minded homolog 1 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Single-minded homolog 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Single-minded homolog 1 antibody is available for research use only.

Storage Conditions

Aliquot the Single-minded homolog 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Single-minded homolog 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human brain tissue with Single-minded homolog 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.