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VSNL1 Antibody / VILIP1 / Visinin-like protein 1

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

P62760

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 47-75 from the human protein was used as the immunogen for the VSNL1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the VSNL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This VSNL1 antibody is available for research use only.

Storage Conditions

Aliquot the VSNL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the VSNL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human brain tissue with VSNL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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